The image data utilized were digital imaging and communications in medicine (DICOM) files that were imported into the Horos program by CDs provided by the neuroradiology department. Volumetric data and extent of resection were obtained via region of interest-based 3D analysis using Horos image-processing software. Preoperative and postoperative tumor volumetric analyses were performed per patient. Preoperative and postoperative tumor analysis are compared after maximal safe resection. Two case reports are presented with step-by-step instructions detailing the region of interest segmentation function, computing volume function, and 3D tumor volume rendering. Video depicting volumetric analysis of high-grade glioma resection in the pediatric population using Horos software. Tumor volumetric analysis guide: Horos software Horos software is a highly effective means of volumetric analysis for the postoperative analysis of residual volume after maximal safe resection of high-grade gliomas in pediatric patients. Horos software provides increased accuracy and confidence in determining the postoperative volume and is useful in assessing the impact of residual volume on outcomes in patients with high-grade gliomas. This is a case series of two pediatric patients with histologically confirmed high-grade gliomas who underwent tumor resection as definitive treatment from June 2011 to June 2019. We present the use of Horos software as a postoperative tool for residual tumor volume analysis in children with high-grade gliomas (HGG). Horos (LGPL 3.0 GNU Lesser General Public License, Version 3) is a free, open-source medical image viewer with a user-friendly interface and three-dimensional (3D) volumetric rendering capabilities.
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